Comparison of the direct agglutination and indirect hemagglutination tests in the determination of blood serum titers to Escherichia coli organisms.

نویسندگان

  • I A Schipper
  • C Kelling
  • H Ebeltoft
  • D Graves
چکیده

A comparison of the direct agglutination test and the indirect hemagglutination test for the detection of blood serum antibodies to Escherichia coli organisms indicated that these serological tests were comparable. In some instances the indirect hemagglutination test provided higher endpoint readings. Preparation of the antigens for the indirect hemagglutination test was more time consuming than for the direct agglutination test. Crude extract and purified polysaccharides were comparable as red blood cell sensitizing agents.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

The role of mannose sensitive and mannose resistant pili of Escherichia coli in urinary tract infection

  In order to study the relationship between hrmolysin / ashesins of Escherichia coli and occurrence of urinary tract infection ( UTI), the capacity of 570 isolated E.coli in agglutination of human or guinea pig erythrocytes and their lysis were tested. These isolates were obtained from the urine of patients with bacteriuria and from the stool of healthy people. Three patterns of hemagglutinat...

متن کامل

Studies on Hemagglutination and Hemolysis by Escherichia Coli Antisera

A study on hemagglutination and hemolysis by Escherichia coli O111 and O55 (rabbit) antisera and on hemagglutination and hemolysis inhibition by E. coli O111 and O55 antigens revealed the following facts. 1. Red blood cells of man, dog, rabbit, guinea pig, sheep, rat, and chicken adsorb E. coli O111 and O55 antigens and thus become specifically agglutinable by the homologous E. coli antisera. 2...

متن کامل

Serological Studies of O Antigens of Escherichia Coli by Means of the Hemagglutination Test.

Kunin, Calvin M. (University of Virginia, Charlottesville) and Mary V. Beard. Serological studies of O antigens of Escherichia coli by means of the hemagglutination test. J. Bacteriol. 85:541-548. 1963.-The serological interrelationships among most of the known O antigens of Escherichia coli were studied by the hemagglutination test. Good agreement with the bacterial agglutination test was foun...

متن کامل

P fimbriation of Escherichia coli strains from patients with urosepsis demonstrated by a commercial agglutination test (PF TEST).

We examined 138 Escherichia coli strains isolated from cultures of blood specimens from urosepsis patients (n = 78) and nonurosepsis patients (n = 30) and from fecal specimens of a healthy control group (n = 30) for P fimbriation with a mannose-resistant hemagglutination test, an indirect immunofluorescence assay, and a new commercial particle agglutination test (PF TEST; Orion Diagnostica, Esp...

متن کامل

A comparison of titers of anti-Brucella antibodies of naturally infected and healthy vaccinated cattle by standard tube agglutination test, microtiter plate agglutination test, indirect hemagglutination assay, and indirect enzyme-linked immunosorbent assay

AIM We determined the antibody response in cattle naturally infected with brucellosis and normal healthy adult cattle vaccinated during calf hood with strain 19. MATERIALS AND METHODS The antibody titers were measured by standard tube agglutination test (STAT), microtiter plate agglutination test (MAT), indirect hemagglutination assay (IHA), and indirect enzyme-linked immunosorbent assay (iEL...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Applied microbiology

دوره 25 3  شماره 

صفحات  -

تاریخ انتشار 1973